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High-Throughput Screening Core

Director: John Cooper, MD, PhD

The High-Throughput Screening Core provides the facility and expertise to aid users in the development and running of screens with siRNA or small molecules.

The core provides access to compound collections and siRNA libraries and maintains facilities optimized for siRNA whole-cell work, chemical screens and microbiology.

The core will allow for automated screening of cells cultured in 96- or 384-plate formats and can be applied to multiple investigator-initiated applications throughout the university community. The applications include:

  1. Small-molecule drug screens applied to cells engineered by Washington University investigators

  2. Small-molecule or peptide screens to identify enzyme inhibitors (directed against high-priority proteases, kinases, phosphatases, etc.) or modulators of protein-protein interactions

  3. Genomewide siRNA library screens against small molecules in mammalian cells targeting cell growth or cell death responses

Platforms incorporating biolumninescence or fluorescence HTS readouts are available, and an HTS core can be coupled with high-content cell screens, such as the automated, computerized Zeiss microscope offered by Cellomics. These systems enable a computer to be trained to recognize morphological changes at the cellular level, allowing for automated screens of changes in cell biological properties.

 

LOCATION: Rooms 366 and 316 McDonnell Sciences Building

PRICING: Please contact the core for current pricing of services offered.

CONTACT: John Cooper at jcooper@wustl.edu, 314-362-9373

As of April 7, 2008, the National Institutes of Health (NIH) requires investigators with a publication using Siteman (or other NIH-funded) shared resources to submit (or have submitted for them) their final peer-reviewed manuscripts to PubMed Central (PMC) upon acceptance of the publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see publicaccess.nih.gov/FAQ.htm#b7 or http://becker.wustl.edu/classes-consulting/specialized-expertise/nih-public-access-policy for more information.

PUBLICATION ACKNOWLEDGEMENT: If research supported by the High-Throughput Screening Core results in publication, please acknowledge this support by including the following in your publication(s):

We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, Mo., for the use of the High-Throughput Screening Core, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA91842.

LINKS: Core Web site