Murine Embryonic Stem Cell Core
Director: Timothy Ley, MD
The Murine Embryonic Stem Cell Core is responsible for:
- Creating and providing state-of-the-art reagents for the production of targeted mutations in embryonic stem cells.
- Creating and maintaining quality-controlled embryonic stem cell lines from a variety of mouse backgrounds.
- Teaching the methodology of embryonic stem cell culture and manipulation.
The mESC Core provides consultative services for Siteman Cancer Center members when they are designing knock-out or knock-in vectors, and it creates and distributes user-friendly plasmids that facilitate the generation of targeting vectors. The core also attempts to assist all laboratories with blastocyst injections if injection facilities are not available to that laboratory. By providing a comprehensive service, the mESC Core facilitates the production of gain-of-function and loss-of-function mouse models for Siteman Cancer Center faculty.
LOCATION: Southwest Tower, Room 702
PRICING: Please contact the core for current pricing of services offered.
TO ACCESS: Contact the Murine Embryonic Stem Cell Core at 314-362-9348, e-mail the facility at firstname.lastname@example.org or fill out the online service request form at http://escore.im.wustl.edu.
As of April 7, 2008, the NIH requires investigators with a publication using Siteman (or other NIH-funded) shared resources to submit (or have submitted for them) their final, peer reviewed manuscripts to PubMed Central(PMC) upon acceptance of publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see http://publicaccess.nih.gov/FAQ.htm#b7 or http://becker.wustl.edu/classes-consulting/specialized-expertise/nih-public-access-policy for more information.
PUBLICATION ACKNOWLEDGEMENT: If research supported by the Murine Embryonic Stem Cell Core results in publication, please acknowledge this support by including the following in your publication(s):
We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, Mo., for the use of the Murine Embryonic Stem Cell Core, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA91842.