Siteman Flow Cytometry Core

Director: Bill Eades
Lab Manager: Jackie Hughes

Staff: Chris Holley and Dan Schweppe

The Siteman Flow Cytometry Core offers services for flow cytometry-based cell analysis and cell sorting. The instrumentation includes five benchtop analyzers and three cell sorters assemblies with a total sorting capacity equal to five traditional cell sorters.

SELF-SERVICE FLOW CYTOMETRY ANALYSIS:

The analysis section of the core offers self-service on five digital FACScans, updated to three lasers and either eight or 10 colors by Cytek Development. FACScan 2 offers the ability to aspirate directly from 96-well microtiter plates. Optics are fixed, and specifications/example fluorophores are described here

Operator training for flow cytometry is required for self-service and available by individual three-hour appointment. FlowJo Collectors Edition is used for acquisition.

Four computer workstations are available in the core for post-acquisition data analysis at no charge. They are offered on a first-come, first-served basis and include FlowJo, Kaluza, Summit and WinList analysis suites. These applications are meant to be self-taught, but training is available by appointment.

STAFF-OPERATED CELL SORTING:

Cell sorting is performed by a highly trained staff that is keenly aware of the physical science surrounding electrostatic sorting. Over 50 years of collective experience from varied specialties converge and include clinical process, sample preparation, and instrumentation design and support. All cell sorters are configured for flexibility. Given proper notice, the staff can set up for esoteric laser wavelengths and emission filter combinations, as well as up to four-way subset sorting and sorting into well plates. Details about specific cell sorters can be found here.

As always, any pathogenic potential of samples must be disclosed, and an IBC protocol for your lab must be produced before any analysis acquisition or sorting will be scheduled.

SAMPLE PREPARATION:

Sample preparation for flow cytometry analysis or sorting is available by appointment based on staff capacity. This service is intended for clinicians with no lab facility or staff who wish to perform research flow or for complex staining to be consistently reproducible, a challenge for newcomers to flow who go directly into multiparameter flow. Antibody stocks maintained by the core are titrated and access-controlled, and clients with their own antibodies must also titrate with viewable results.

FEE SCHEDULES:

Siteman member rates are for research and research associate members only. Inquiries regarding cancer center membership should be directed to the Siteman Cancer Center's research administration office at 314-454-8110.

Current Fee Schedule  

LOCATION: Southwest Tower, Rooms 703, 704 and 704A

CONTACT: Call 314-362-9364 or e-mail sitemanflow@wustl.edu

LINKS: Instrument scheduling calendars

As of April 7, 2008, the NIH requires investigators with a publication using Siteman (or other NIH-funded) shared resources to submit (or have submitted for them) their final, peer-reviewed manuscripts to PubMed Central (PMC) upon acceptance of publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see http://publicaccess.nih.gov/FAQ.htm#b7 or http://becker.wustl.edu/classes-consulting/specialized-expertise/nih-public-access-policy for more information.

PUBLICATION ACKNOWLEDGEMENT: If research supported by the Siteman Flow Cytometry Core results in publication, please acknowledge this support by including the following in your publication(s):

We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, Mo., for the use of the Siteman Flow Cytometry Core, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA91842.