Director: Timothy Graubert, MD
Lab Manager: Bill Eades
Staff: Jackie Hughes, Chris Holley
The High-Speed Sorter Core Lab offers services for flow cytometry based cell analysis and cell sorting. The instrumentation includes four benchtop analyzers and three cell sorters with a sorting capacity equal to 7 traditional cell sorters.
The analysis section of the core offers self service on flow cytometry analyzers manufactured by B-D Biosciences and modified to two lasers and 5 fluorescent detectors by Cytek Development. One of these instruments offers the ability to aspirate directly from 96 well microtiter plates. Intracellular cytokine and phospho-flow analysis is also available using a BioRad/Luminex platform. Operator training for flow cytometry is required for self service and available by individual appointment. Off-line data analysis resources are available at no charge, including CXP, CELLQuest Pro, FlowJo, and WinList.
The sorting section of the core offers staff-operated flow cytometric cell sorting, experiment design aid and sample preparation reference. Model organism material with no pathogenic potential can be sorted in our basic research facility. It houses a new iCyt Reflection, a four sort channel, ten detector per channel, six laser wavelength sorter. This new sorter technology will allow for either four independent sort experiments to occur simultaneously, or a single sample to be taken to parallel sort channels (up to two) for extreme rare event sorting. The instrument also shows enhanced scatter and fluorescence sensitivity through reflective optics.
A traditional single channel cell sorter, a Dako MoFlo with 10 detectors and 3 lasers is available for human cell study in a BSL3 containment suite. Any pathogenic potential of samples should be cited for proper appraisal and handling methods before appointments may be created.
The sorting core will soon offer GMP grade cell sorting for human study and therapy, the staff operated process to be performed in a 280 sq.ft. class 10,000 clean room with positive pressure. The sorter is being developed with iCyt Mission Technology of Champaign, IL., and is a GMP specific design expansion of the Reflection research sorter used in our general research lab for model organism study.
LOCATION: Southwest Tower, Rooms 703, 704, and 704A
CONTACT: Phone (314) 362-9364 or email eadesb@wustl.edu
As of April 7, 2008, the NIH requires investigators with a publication using Siteman (or other NIH-funded) shared resources to submit (or have submitted for them) their final, peer reviewed manuscripts to PubMed Central(PMC) upon acceptance of publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see http://publicaccess.nih.gov/FAQ.htm#b7 or http://becker.wustl.edu/services/scholarly/nihpolicy.html for more information.
PUBLICATION ACKNOWLEDGEMENT: If research supported by the High Speed Cell Sorter Core results in publication, please acknowledge this support by including the following in your publication(s):
We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, Mo., for the use of the High Speed Cell Sorter Core, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA91842.
LINKS: Instrument scheduling calendars. (NOTE: the scheduling calendars are only accessible from computers within Washington University)