Siteman Flow Cytometry Core Self-Service Analysis 

The analysis section of the core offers self-service on five digital FACScans, updated to three lasers and either eight or 10 colors. FACScan 2 offers the ability to aspirate directly from 96-well microtiter plates. Optics are fixed, and specifications/example fluorophores are as follows:

FACScans 1-4 (10 color)

  • Blue laser (488nm excitation) FL1 (530nm emission centering wavelength/30nm bandwidth)- FITC, GFP, YFP, CFSE
  • Blue FL2 (585/42)- PE, Propidium Iodide
  • Blue FL3 (620/20)- PE/Texas Red, ECD
  • Blue FL4 (695/40)- PerCP/Cy5.5, 7-AAD
  • Blue FL5 (740 LP)- PE/Cy7
  • Red laser (637nm) FL1 (660/20)- APC, Alexa 647
  • Red FL2 (725/20)- Alexa 700
  • Red FL3 (740 LP)- APC/Cy7, e780
  • Violet laser (407nm) FL1 (450/50)- Pacific Blue, Brilliant Violet 421
  • Violet FL2 (545/30)- Pacific Orange, Krome Orange, Live/Dead Yellow


GFP/YFP FACScan (eight color)

  • Blue FL1 (510/20)- Green Fluorescent Protein variants
  • Blue FL2 (550/30)- YFP
  • Blue FL3 (695/40)- PerCP/Cy5.5, 7-AAD
  • Blue FL4 (740 LP)- PE/Cy7
  • Red FL1 (660/20)- APC, Alexa 647
  • Red FL2 (740 LP)- APC/Cy7, e780
  • Violet FL1 (450/50)- Pacific Blue, Brilliant Violet 421
  • Violet FL2 (545/30)- Pacific Orange, Krome Orange, Live/Dead Yellow


LINK:

Instrument scheduling calendars

Inquiries regarding flow cytometry services should be directed to 314-362-9364 or sitemanflow@wustl.edu.